Partially Purification M Protein from Streptococcus Pyogens and Molecular Study of Some Virulence Factors

Abstract

The current study was conducted to isolation one type of surface protein of Streptococcus pyogens this protein separated using partially purification method. Purifiction  process include precipitation of crude  M protein  one peak of this protein was appeared, the best precipitate rate was 30% . PCR assay were performed to identify the presence of some genes related to pathogenicity in bacteria responsible from Tonsillitis .Streptococcus pyogens represented the largest causative agent of inflammation of the tonsil so the study revealed the presence of certain gene in some isolate of bacteria, including the gene SpeB that gave large proportions in the study isolates 12 out of 37 at a rate of (32.4%).

Keywords : S.pyogens , Tonsillitis , M protein ,PCR ,SpeB