Effect of Insulin Transferin Selenium (ITS) on oocyte maturation in vitro in Indonesian goats

Abstract

The aim of the present study was to evaluate the effects of Insulin-Transferin-Selenium (ITS) on maturation of oocytes in vitro in Indonesian Goats. Goat ovaries were collected at a local slaughterhouse and washed twice in sterilized NaCl solution. The follicular content including cumulus-oocyte complexes (COCs), were collected by aspirating the visible small antral follicles (around 2-6 mm diameter) with a 10 ml syringe equipped with an 8-gauge needle. The oocytes with more two layers were cultured for 28 hours at 38.5^oC and 5%CO₂. After maturation the cumulus cells were removed and the denuded oocytes were stained with 2% aceto-orcein to determine the nuclear oocyte status. The result showed that the rate of nuclear maturation in ITS-treated groups was significantly higher (P<0.05) than in the ITS-untreated group, were 38.14%^a;  61,40%^b and 71,55%^b ,  respectively for medium without ITS,  5 ng/ml ITS and  10 ng/ml ITS.  This observation indicated that supplementation of ITS into culture medium was effectively enhance oocyte nuclear maturation in vitro.

Keywords:  oocyte, nuclear maturation, Insulin Transferin Selenium, in vitro maturation