Extractability of Thevetia Peruviana Glycoside using Various Organic Solvents

Abstract

This work was designed to investigate the suitable organic solvent(s) for the effective removal of Thevetia peruviana glycoside in the seed. The anti-nutritional status of the seed was also assessed. Of all anti-nutrients screened (Tannins, Phlobatannins, Saponin, Flavonoids, Cardiac glycoside and Lectins) in the seed meal, only Cardiac glycoside (at 7.98 w/w % of the seed meal) was found. Attempt to remove the cardiac glycosides content using various organic solvents (Methanol, Ethanol, Ethyl acetate, n-hexane, n-butanal, Chloroform and Acetone) shows that 80% methanol/ethanol solvent mixture (8:2) reduces the toxic cardiac glycoside from the seed meal optimally.

Keywords: Thevetia peruviana seed, cardiac glycoside, aglycones, detoxification