Role of RhIR and RhII Genes in Biofilm and Multidrug Resistance of Burn Isolated Pseudomonas aeruginosa.

Abstract

During the period  of 3 months in Baghdad  Teaching hospital  from March to June 2014 ,(24 )isolates of Ps. aeruginosawere assumed from burn infection.Further identification were done by using rpsL gene as house keeping gene by PCR Ps. aeruginosa. isolates from Baghdad hospital that identified by PCR method, antibiotic sensitivity had done by Kerbey Bauer Disk method for (10)antibiotics which showed that the highest resistance (100%)(n=24) to Tetracyclin, Chloramphinicol,Ceftazidime, Cefoxitin and Ceftriaxone, while the resistance to the Cefepime(83.33%)(n=20), Pipracillin (62.5%)(n=15),the same resistance to Ciprofloxacin and Tobramycin (50%)(n=12), Azithromycin (41.66%)(n=10).Results of biofilm formation which detected by TCP Method showed that (66.66%)(n=16) of isolates had high biofilm production most of them are male isolates while the most of moderate production was in female isolates.The highest presence of  quorum sensing genes was for RhIR gene (83.33%)(n=20) while RhII genewas (58.33%)(n=14).Through these results it was observed that there was not any effect of quorum sensing genes on biofilm formation or multidrug resistance while showed relationship between biofilm production and multidrug resistance of negative isolates for quorum sensing  genes.