Diversity of Quantitative Characteristics and Growth Hormone Gene of the Local Thin-tailed Sheep in Some Districts at Jambi Province

Abstract

This study aims to determine the diversity of quantitative characteristics and growth hormone gene of thin-tailed sheep (DET) in several districts/cities in Jambi province. There are two stages of this researchis,  the field and laboratory. Data collected in the field was characteristic of quantitative: wither height (WH), body length (BL), chest grid (ChG), chest deep (ChD), the width of the chest (ChW), body weight (BW), Body weight gain (BWG) and blood samples. The total number of samples of 160 tail aged  1-2 years (I1 = a pair of permanent teeth). Research in the laboratory include : DNA isolation, amplification and gelpurification using Mspl restriction enzyme by PCR-RFLP.

Data analysis includes, frequency of genes, heterozygosity values, Informative Polymorphic Content and the balance of GH gene genotype. Differences in quantitative characteristics between districs/cities among Genotypes GH gene fragment MspI and AluI was analyzed using t-test

Analysis of the data include : the frequency of the gene, the value of heterozygosis, Informative Polymorphic Content (PIC) and the balance of GH gene genotype. Quantitative characteristic differences among districts / cities as well as the quantitative characteristic differences between genotypes Fragments MspI and AleI GH gene were analyzed using t-test.

The present study concluded that : 1) there is no differences in quantitative characteristics   both female male   between Sungai Penuh City and  Kerinci , as well as between Muaro Juambi dan Batanghari District 2) there is  polymorphism of GH gene  on the TTS highlands and lowlands in Jambi Province. 3) quantitative characteristics of  TTS  in the highlands and lowlands associated with genotype frequency of gene GH MspI and AluI.

The conclusion of this study were 1) Quantitative characteristics both male and female between Kerinci with Sungai Penuh City is no different, between Muara Jambi and Batanghari District no different but between Kerinci and Sungai Penuh City and Muara Jambi and Batanghari is different; 2) The marker of -RFLP MspI and AluI showed there is polymorphism of GH gen of  thin-tailed sheep in all selected lodation; 3) Characteristics of quantitative of thin-tailed sheep in all districts/cities associated with genotype frequencies of GH gene MspI and AluI.

Keywords: thin-tailed sheep, phenotype diversity,  growth hormone gene (GH), PCR-RFLP.