Evaluation the Non-Thermal Plasma Application Activity in AFB1 Detoxification

Abstract

Contamination raw agricultural materials has been a food safety concern for human and animals. A non thermal plasma or cold plasma  is a novel antimicrobial intervention, that can be reduce the level of aflatoxin B₁(AFB₁) in complete cow's feed samples .AFB₁ are carcinogenic compound produce primarily by two certain strain of Aspergillus include A. flavus and A. parasiticus , the  contamination of feed is arise for  animals health. Fifty samples from complete cow's feed were designated into imported 15 samples and local 35 samples , obtained randomly from different region in Baghdad from March 2014 to February 2015. Samples were tested for AFB₁ by ELISA and HPLC technique and exposure to application cold plasma protocol to treatment of AFB₁ contamination the samples in different time (5, 10 and 15) seconds at 3.5 cm between the plasma source and samples then tested by ELISA and HPLC. There are appears the best time successful in reducing levels of toxin at 10 sec. in local samples 3.12, 0.05 ng / g imported samples 1.21, 6.19 ng / g in HPLC and ELISA. In local and imported samples at 10 sec and that was 15 sec less time periods ability to reduce toxin level in local and imported samples, that indicated the length of exposure to NTP application is not necessary to reduce toxin level. According to the study we observed that the results from ELISA method were more sensitive, accuracy and simplicity when compared with results from HPLC technique.

Keyword: Cold plasma, decontamination,AFB_1, HPLC, ELISA.