In Vitro Regeneration of Tomato (Lycopersicon esculentum Mill.) Role of Metatopolin, Benzyladenine and Casein hydrolysate

Abstract

The effect of adding metatopolin (mT), benzyladenine (BA) individually to the nutrient medium at equal molar concentration (1 mM) and their interaction with three concentrations (0.1, 0.3 & 0.5 g.l^-1) of casein hydrolysate (CH) on in vitro regeneration of Tomato (Lycopersicon esculentum Mill.) cv. Super marmande were studied. mT caused a significant increase in leaves number and adventitious shoots number compared with BA, while BA exceeded mT significantly in shoots length.The results show that there were no significant differences between mT and BA regarding roots number and root length. Both BA and mT caused induction of callus at the base of microshoots. CH at 0.1 g.l^-1 and its interaction with mT caused a significant increase in leaves number and adventitious shoots number whereas its interaction with BA caused a significant increase in shoot length. Increasing CH concentration and its interaction with either mT or BA caused a gradual significant decrease in callus fresh weight when compared with the control.

Keywords: Metatopolin, Benzyladenine, Casein hydrolysate, Tomato, Adventitious shoots, Roots, Callus.