Epidemiology of Ovine Pasteurollosis in Lume District, East Shewa Zone of Oromiya Region, Ethiopia

Abstract

The study of ovine pasteurellosis was conducted in Lume districts, East Shoa Zone of Oromia region, Ethiopia to determine the prevalence of Mannheimia haemolytica, Pasteurella trehalosi and Pasteurella multocida from nasal swabs (384), abattoir specimens (145), and the serotype diversity among the species from sheep sera (150). A total of (115) isolates of M. haemolytica, P. trehalosi and P. multocida were isolated from nasal swabs of apparently health and clinically sick sheep and from pneumonic lungs. The M. haemolytica, P. trehalosi and Pasteurella multocida was isolated from the nasal swabs (11.2%), (7.6%) and (2.1), whereas M. haemolytica isolates from pneumonic lungs (11.7 %), P. trehalosi (10.3) whereas Pasteurella multocida was the lowest among species isolated (2.1 %). The overall isolation rate of M. haemolytica and P. trehalosi and Pasteurella multocida was 15.7%, 11.5% and 2.9 respectively. From 145 lung samples collected and cultured, Pasteurella was isolated successfully in 35 (24.1%) sheep.Out of 35, the percentage recovery rate of M.haemolytica 17(11.7%), P.trehalose 15(10.3%) and P.multocida 3(2.1) % from the lung lesion sample respectively. On the basis of these results M. haemolytica and P.trehalose was the most common cause of pasteurellosis in sheep at Lume district. A total of 150 sheep sera were examined for serotype specific antibodies using indirect haemagglutination test for M. haemolytica, P.trehalose and P.multocida serotypes. Variation in prevalence among the different serotypes was observed (P< 0.001). The IHA test revealed that serotype A1, A2,A7,T3,T10,and T15 were the dominant serotypes with 23.3%, 42.6%,32,51.3,29.3 and 30% positive by IHA whereas serotypes PAs (P.multocida biotype A) and T4 were the least positive with 14.6% and16% respectively. Generally Both bacterial and serological results of this study showed that the causal agents of pasteurellosis are prevalent in the area, and serotypes A1,A2, A7, T3,T10 and T15 were dominant over the other serotypes.

Keywords: Mannheimia and pasteurella spp, nasal swabs Pasteurella,isolation, serotypes,IndirectHaemagglutination(IHA),Lume(EastShewa).