Molecular Analysis of CMV Genes Variation in Pregnant Woman

Abstract

In these papers were studied molecular for both, molecular detection of human Cytomegalovirus (hCMV) and genotyping based of gB and gN genes in pregnant women suffered from previous abortion by means of Immunoglobulin M and G of Cytomegalovirus antibodies and nested polymerase chain reaction for universal detection of beta herpers viruses for amplification in envelope glycoproteins of Cytomegalovirus of  ̴ 800 bp DNA segament encoding for glycoprotein B (gB) gene and glycoprotein N (gN) gene were investigated in 30 pregnant women in Al-Batool Maternity hospital, Wasit provence, Iraq, whom suffered from previous abortion during from October 2014 to October 2015. Showing through it thirty women had increased titers of IgM and IgG types of Cytomegalovirus antibodies. This work includes a molecular study for both, molecular detection of HCMV and for genotyping based of gB and gN genes. Nested PCR technique was adopted as described previously for universal detection of beta herpes viruses for amplification of ~800bpDNA segment encoding for  glycoprotein B(gB) gene (UL55) was carried out. To determine the genetic polymorphisms in envelope glycoproteins of CMV, glycoprotein B (gB) gene, and glycoprotein N (gN) gene, have been amplified. Thus, we conclude homology of gN sequence.  gN-based  genotyping study reveals three types of gN1, gN3, gN4 genotypes. This study showed that gN 1 genotype are more frequent genotype in patient sample followed by gN4 type, and one case with gN3 genotype.

Keywords:- hCMV, glycoprotein B (gB) gene, glycoprotein N (gN) gene, Nested PCR technique