Survey of occurrence of bacteria Vibrio cholera in the two provinces Baghdad and Babel

Abstract

The present study was branched into two lines ,the first line aim to study Tigris River within Baghdad city  and Euphrates  River within Babel city to assess physical ,chemical and biological factors of  rivers water that Affect  the quality of water . the second line is isolating and diagnosis of  Vibrio  cholera bacteria from clinical and environmental sources in the two provinces and study of its  sensitivity against (13) antibiotic also conducting a molecular study to detect  the presence of plasmids and the toxin genes in All  isolated bacteria . the study area included three stations on Tigris river in Baghdad city and three stations on Euphrates river in Babel city , monthly samples were collected from October 2015 to September 2016 in addition to samples were collected from patients in three Hospitals  by using sterile containers .

Keywords : Choleragen , Primers , DNA agarose gel electrophoresis