Invitro Antimicrobial Activity of Plumbago Zylanica L.

Abstract

Beside the search for new plants; Plants investigated to have medicinal value for particular diseases has been also checked for extra pathogens and further in other  biological activities in a search for efficient and effective drug. In this study the in vitro antimicrobial capacity of Plumbago zeylanica L. root extracts were investigated against seven bacterial strains and one fungal strain. Antimicrobial activity was done   employing Agar disc diffusion and Minimum inhibitory concentration (MIC) was determined involving broth dilution method. The chloroform crude extracts as well as fractions were more potent than ethanolic extracts.  From MIC findings S. entritidis, S. aureus , E.coli were withdrawn from growth at  0.2mg/mL,  1mg/ml and 2mg/ml respectively. In addition, nine pure compounds were isolated and one (PFr₈) inhibited a bit higher than the standard drugs.

Keywords: Plumbago zeylanica L, antimicrobial activity, minimum inhibitory concentration (MIC), fractionation, invitro test