Molecular Barcoding and Phylogeny Reconstruction of Rhynchoporus sp in Minahasa North Sulawesi Based Partial Cytochrome Oxidase Sub Unit 1 Gene (CO1)

Abstract

Molecular phylogeny reconstruction of Rhynchophorus sp from Cocus nucifera L, Arenga pinata and Metroxylan sagu was investigated using partial sequences of Cytochrome c oxidase subunit I gene (CO1). Three type of  Rhynchophorus sp according the place of live used this study. Rhynchophorus sp from Cocus nucifera (AR1), Metroxylan sagu (SG1) and Arenga pinata (AR1) was analysis the partial CO1 gene. Phylogeny trees was constructed using MEGA 6,0 and Geneous 6,0. From the results of this studied, based on partial CO1 gene, Rhynchoporus living at the Arenga pinata is Rhynchoporus palmarum while Rhynchoporus living on Sago palm (Metoxylan sagu) and  Coconut (Cocos nucifera) are Rynchonporus vulneratus. The results of this study is the first step of the revision of the uncertain taxonomic status and phylogenetic relationships among the Rhynchophorus genus in Minahasa, North Sulawesi. Further analysis should be performed using other Rhynchophorus species as well as other molecular markers.

Keywords: Rhynchophorus sp, CO1, Cocus nucifera, Arenga pinata, Metroxylon sagu Rottb, CO1, phylogeny tree