Deletion of introne-4 of Endothelial nitric oxide synthase gene in Behcet’s disease-Iraq .Ali alkazzaz* Medical college- Babylon University

Abstract

Behcet’s disease (BD) is multi systemic inflammatory disorder of unknown cause, it characterized by recurrent oral and genital ulcer, skin lesion and uveitis. The etiopathogensis of Behcet’s Disease had long been postulated, possibility that immunological abnormalities induced by microbial pathogens in genetically susceptible individual are important in the pathogenesis. There was no positive association between eye involvement, arteritis and HLA5 tissue typing and positive pethergy test .The HLA5 was positive in 62% of patients with Behcet’s disease in Iraqi patients. Endothelial nitric oxide synthase is synthesized by arginine oxidation, it is one of nitric oxide synthase family, this enzyme has three isoform tow constitutive (neuronal NOS include nNOS and NOS-I)and one inducible NOS (Inos, nos-II), endotheline enzyme responsible of generate small amount of nitrous oxide, The eNOS gene is located on chromosome 7q35-36. 33 Iraqi patients with Behcet’s disease compared with the same number of healthy population and it was conducted in Merjan teaching hospital in Babylon –Iraq in rheumatology unit from same ethnic Arabic group. The genotype study was done for both groups in the biological department of Science Collage in Babylon University during 2013. Genomic DNA was extracted from peripheral blood leukocytes using standard protocols. PCR experiment was performed using Forward primer 5-AGGCCCTATGGTAGTGCCTT-3 and revers primer 5- TCTCTTAGTGCTGTGGTCAC-3 in (clever scientific thermo cycler PCR). The study aims to detection deletion in of introne-4 of Endothelial nitric oxide synthase gene in Behcet’s disease, the total number of patients was 33 patients, 11[33.3 %] were female and 22 [66.6%]were male, the  mean age of the patients was (34.45± 11.51)years. (69.7%) of patients did not had family history of Behcet’s disease, (57.6%) of patients were less than 5 years having the disease.57.6%of patients were got no active disease at time of study and 42.3% got active disease and there was significant association with genital involvement , central nervous system and disease duration with disease activity .  The genetic study revealed that no significant related of deletion of Endothelial nitric oxide synthase gene in Behcet’s disease Iraqi patients.

Keywords: Behcet’s disease [BD], Iraq, Endothelial nitric oxide synthase[e NOS], PCR.