Biogeography and Polyphasic Approach of Pseudomonas strains from Agriculture land of Madhya Pradesh

Abstract

The present study related to microbial isolation of Pseudomonas species from different district of Madhya Pradesh helps to conclude bacterial species with similar biochemical property having approximately common genetic content exhibit molecular diversity to evaluate and compare the new Miniprimer-PCR to the two DNA-based typing techniques and the phenotypic characteristics for the purpose of assessing the diversity of microbial community isolated as collection of fluorescent pseudomonads. All the 50 strains of pseudomonas sp such as Pseudomonas aeruginosa, P.putida, P. fluroscence, and P. streuzi were shown to have genetic similarity with variation in arrangement of nucleotides in genome. Expression of species within the genus is regulated by reading frame of organism, therefore, with common concentration of DNA most of the species shows architectural motive once NTSYS prediction were made using UPGMA .Ornamentation of genome represent base substitution thus defining evolutionary distance along with constancy of character within species. All the Pseudomonas isolated looking into their biochemical profile can be used as PGPR and biocontrol agents.

Keywords: PCR , DNA,UPGMA Microbial diversity genetic similarity.