The Antimicrobial Effect of Diode Laser with Heat Producing toward E.Faecalis in Root Canals and in Periapical Lesions

Abstract

In our study we tested the efficacy of disinfection with diode laser Nd : YAG root canals of single-rooted teeth and lesion models of different sizes (6, 10 and 14 mm), in which we put the teeth and made?? an in vitro model. Teeth and models we re-infected with E. faecalis, as one of the most common microbes for re-infection, till the growth of the biofilm. The growth of the biofilm was confirmed microbiologically on agar plates. Viability of microorganisms in the samples after laser treatment and compared to samples without treatment, were analyzed with a fluorescence method by flow Cytometry. Therapy with diode laser Nd : YAG showed a significant increase in the percentage of dead cells compared to the control group. At the same time we have found a significant difference between the different dimensions of periapical lesions. The smallest dimension lesions of 6 mm showed the highest number of dead cells.

Keywords: Periapical lesions, diode laser Nd : YAG, root canal disinfection