Evaluation of Inhibitory Activity of the Aqueous & Alcoholic Extract of Citrullus Colocynthis towards Staphylococcus Aureus

Abstract

Number of chemical and alcoholic and aqueous extracts have been prepared from four parts of Citrulluss Colocynthis which is the study focus that includes seeds, core, shell and fruit. The primary chemical diagnosis results show that the plant parts of the Citrullus Colocynthis of aqueous and alcoholic extracts contain the active ingredients, such as sSponins, Flavonoids, Coumarins, Resins, Alkaloids, Tannins, Phenols , Glycosides and PH rangd between 5.34-5.63 with LSD=(P<0.05). All extracts for each part of plant were tested or examined for antimicrobial activity toward Staph. Aureus from different cutaneous infections (S1, S2, S3, S4, and S5). The results showed that antimicrobial activity depend on kind of extract and tested microorganism. The inhabitation zone of aqueous extracts of dried parts of plants against S1 ranged between 5 mm for core and fruits, 9mm for  seeds and 10mm for shell with LSD=(P<0.05), while S2 ranged between (3-7) mm for fruits and seeds respectively with LSD=(P<0.05), S3 (1-13) mm for shell and seeds respectively with LSD=(P<0.05), S4 (1-5) mm for fruits and shell with LSD=(P<0.05), and S5 (0-9) mm also for fruits and shell respectively with LSD=(P<0.05). While alcoholic extracts for dried parts of plant against S1 ranged between (10-5)mm for all parts of plants, S2(5-15), S3(10-15) mm. S4(5-10)mm and S5(5mm) for all parts with LSD=(P<0.05). while fresh alcoholic extracts for all parts of plant against  S1(6mm) for core and (4mm) against S5 for core with LSD=(P<0.05). te sage fresh alcoholic extract was not active at different Staph. Aureus with LSD=0.

Keywords: Citrullus Colocynthis, methanolic extract, aqueous extract, biological activity.