Rapid Identification of Human Adenoviruses and Cytokine Estimation among Patients with Epidemic Keratoconjunctivitis in Babylon Governorate, Iraq

Niran Kadhim F. AL-Rubaey, Azhar Amran L. AL-Tahab, Ghanim Aboud Al-Mola


One hundred seventy five conjunctival swabs were collected from patients with keratoconjunctivitis. All swabs were cultured on Blood agar and MacConkey agar , 82 positive bacterial culture isolates were excluded and 93 negative bacterial culture isolates were taken as a study samples. All 93 swabs were subjected to rapid immunochromatography test (IC) test. The results of rapid identification revealed that 79 specimens (84.9%) were found positive for human adenoviruses , whereas 14 specimens (15.1%) were found negative for human adenoviruses. In addition , the results indicated that the positive rate of the IC test for specimens obtained within 1–5 days after of the onset of illness 59 (74.7%) were significantly higher than that for specimens obtained within 6–10 days after of the onset of illness 20 (25.3%) at P-value < 0.05.Tear samples were also collected from both (30) patients and (25) controls to estimate the tear levels of some immune parameters Interleukine-6  (IL-6), Interlukine-8 (IL-8), and tumor necrosis factor alpha (TNF-α) by ELISA (Enzyme linked Immunosorbent Assay) method .The cytokine profile showed that there is a significant increase (P<0.05) in the mean tear concentration of each (IL-6, IL-8 and TNF-α) between EKC patients and control groups , and the result showed that IL-8 was the predominant cytokine released and followed by IL-6 and TNF-α .

Keywords: Adenovirus, Keratoconjunctivitis, Immunochromatography test, Cytokine

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ISSN (Paper)2224-7181 ISSN (Online)2225-062X

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