Ameliorative Potential of Methanolic Extract of Clerodendrum splendens Leaf Against Stress Assault

Abstract

Physiological stress leads to the compromising of the antioxidant status of the biosystem and progressing to the condition of oxidative stress. Thus, this study investigated the attenuating tendency of methanolic leaf extract of Clerodendrum splendens against physiological assault, by evaluating its effects on the oxidative stress markers and hepatopathic state. The assessment of the antioxidant potentials at 500mg/ml was typified using the total antioxidant capacity, and ABTS, DPPH and hydroxyl radicals scavenging activities. Others are reducing power, metal chelating and lipid peroxidation inhibition activities. Activities of Clerodendrum splendens leaf extract were evaluated on hepatophatic indices (ALT, ALP, AST and GGT, tatal bilirubin) as well as antioxidant status (glutathione transferase and superoxide dismutase, activities, and reduced glutathione). The total antioxidant activities of the extract was 149.97±1.233 mg/g AAE (Ascorbic Acid Equivalent). The extract exhibited significant, potent and appreciable antioxidant activities. The study confirmed that the extract contained highly antioxidative bioactive compounds contributing to its antioxidant and anti-inflammatory (anti-stress) potencies.

Keywords: Hepatopathy, Anti-stress, Clerodendrum splendens leaf extract, Antioxidant