Frequency and Comparison of Each icaA and icaD gene Sequences in Bacteria isolated from Otitis Patients

Abstract

Biofilm formation is a part of the pathogenicity by allowing the bacteria to colonize and multiply within the host. Different bacterial species (n=43) of otitis were tested for iacAD genes, then compared by sequencing these genes. All isolates showed positive to icaA gene and / or icaD gene with the frequency of 88.4% for each gene. Serratia marcescens, Providencia vermicola, P. stuartti, Bacillus subtilis, Enterobacter asburiae, E. cloacae, Corynebacterium amycolatum, Bordetella trematum, Proteus mirabilis, P. penneri, Lysinibacillus fusiformis, Pseudomonas putida and Enterococcus faecalis were appeared to have ica gene for the first time. Therefore, the investigation to own ica gene should be in regardless of a selected bacteria. icaD gene showed many mutations in its sequence among five isolates changing a number of amino acids. So, four strains of S. aureus and one Serratia marcescens were recorded as new within the GenBank. ica gene is not confined to certain genera of bacteria, however is diffused among strains, particularly pathogens. Generally, ica genes have many various alleles among the bacteria revealing to give new serious of illness strains.

Keywords: icaAD, sequence, otitis, mutation, alleles