In Vitro Antibacterial Activity of Rumex nervosus and Clematis simensis Plants Against Some Bacterial Human Pathogens

Abstract

Due to quick growth of resistance and high cost of new generation antibiotics, lots of efforts were made to discover new antimicrobial agents from various sources. So, current study was assessed antibacterial activity of ethanol, methanol, acetone, diethyl ether and hexane leave extracts of Rumex nervosus and Clematis simensis by used paper disc diffusion and broth dilution procedures against six human pathogenic bacterial strains. The pathogenic bacteria were Shigella dysenteriae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi and Klebsiella pneumoniae were susceptible to ethanol, methanol and acetone extracts of the leaves of Rumex nervosus followed by Clematis simensis, but hexane extract didn’t displayed any activity. The extreme inhibition zone of 16.3±0.57mm was detected against E coli by ethanol extract of Rumex nervosus and MIC of 3.125mg/ml against Escherichia coli and Shigella dysenteriae by methanol extract. The methanol extract of Clematis simensis formed a marked inhibition of 13.1±0.37mm against Escherichia coli and ethanol extract of Clematis simensis displayed activity against Shigella dysenteriae 14.4±0.45mm and MIC of 6.25mg/ml against Salmonella typhi. Four dissimilar antibiotics like Ciprofloxin, Tetracyclin, Kanamycin and Chloramphenicol were used as standard for tested antibacterial activity against six different human pathogens. The activities were recognized the presence of some secondary metabolites existed in the tested floras which have related with antibacterial activities.

Keywords: Antibacterial activity, Clematis simensis, Human pathogens, Rumex nervosus

DOI: 10.7176/ALST/72-02

Publication date:March 31^st 2019