Acute Toxicity and Antidiabetic Studies of Adansonia digitata Stem Bark Extracts on Streptozotocin Induced Diabetic Rats

Abstract

The study investigates acute toxicity and anti-diabetic potential of solvents extract (n-hexane, Chloroform, Ethyl acetate and Methanol) of Adansonia digitata stem bark. For each solvent, twelve (12) rats were used for Oral LD₅₀ determination, and were grouped into four (4) groups of three rats (3) each. The first three groups were administered with 10 mg/kg, 100 mg/kg and 1000 mg/kg body weight of the extract respectively, while the last group was subdivided into three groups of one rat each and were administered with 2500mg/kg, 3500mg/kg and 5000mg/kg body weight of the extract respectively. Thirty (35) rats were used for the diabetic study and were grouped into seven (7) groups of six (5) rats each. Group I served as normal control, group II served as diabetic control while Groups III, IV, V, VI and VII were induced with diabetes and administered with standard drug, n-hexane, Chloroform, Ethyl acetate and Methanol extract respectively for two weeks. The research found the oral LD₅₀ of all the extract to be greater than 5000mg/kg indicating that the extract was practically non-toxic. Administration of the extract to test groups shows a significant (p<0.05) decrease in blood glucose level in chloroform, ethyl acetate and methanol extract administered groups compared to diabetic control with ethyl acetate extract having the highest activity. It also ameliorate liver toxicity by decreasing the serum level of liver enzymes. Thus indicating a hypoglycemic activity by the extract,

Keywords:Acute toxicity; Adansonia digitata; Antidiabetic; Solvents Extract and Streptozotocin.

DOI: 10.7176/ALST/89-04

Publication date:August 31^st 2021