Protective activity of some antioxidant plant against hydrogen peroxidase genotoxic effects on human lymphocyte

Mufeed Ewadh, Haider Zaidan, Zahraa Haleem, Mona Al-Terehi, Ali Al Saadi


The study aims to evaluation protective activity of different plants  extracts, methanol extract of Barley (Hordeum vulgare L.); sage (Salvia officinalis L) and  Pomegranate cortex  (Punica granutum) and cold and hot water extract  of Ginger ( Zingiber officinale) and soaking method for saffron (Ccinerocus sativus) against oxidative stress caused by H2O2 on human lymphocyte in vitro using DNA fragmentation test . Plant extracts were characterized using thin layer chromatography and detection phenol compounds using ferric chloride reagent. Results showed that some plant extract consist of different compounds and have phenolic compounds in its extracts, some of this extracts didn’t have any effect on DNA when it use alone, but others have genotoxic effects, also its appeared protective effect against H2O2 which it caused fragment in DNA.

Key words: oxidative stress, DNA fragmentation, antioxidant activity.

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ISSN (Paper)2224-7181 ISSN (Online)2225-062X

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