An Analytical and Clinical Study for Measure the Bioavailability of Zinc in Serum Using Flame Atomic Absorption Technique

Abstract

An analytical and clinical study has been applied for measure the bioavailability of Zinc in serum of twenty adults healthy volunteers, using flame atomic absorption spectrophotometer (FAAS) at 213.9 nm. The calibration graph is linear in the ranges of 0.25-1.5 µg.mL^-1 with correlation coefficient (R) 0.09996)µg.mL^1-and molar absorpitivites 22957.76(L.mol^1-cm^-1.The concentration of Zinc determined in serum of all volunteers before and after administered orally a tablet of 50 mg zinc sulphate, produced by Samara drugs company (SDI). All data were subjected to statistical analysis by calculating accuracy, precision in addition to other parameters. The results indicate that the average maximum concentration (C-max ± SD) of blood zinc was 0.8275  ± 0.0511mg.mL^-1after one hour of absorption (T-max ± SD), however one capsule has drug bioavailability through all day.

Keywords: Analytical, Clinical, Bioavailability, Zinc, Flam atomic abortion.