Effect of Insulin Transferin Selenium (ITS) on oocyte maturation in vitro in Indonesian goats

Sri Firmiaty, G. Ciptadi, S. Wahjuningsih, S. Suyadi


The aim of the present study was to evaluate the effects of Insulin-Transferin-Selenium (ITS) on maturation of oocytes in vitro in Indonesian Goats. Goat ovaries were collected at a local slaughterhouse and washed twice in sterilized NaCl solution. The follicular content including cumulus-oocyte complexes (COCs), were collected by aspirating the visible small antral follicles (around 2-6 mm diameter) with a 10 ml syringe equipped with an 8-gauge needle. The oocytes with more two layers were cultured for 28 hours at 38.5oC and 5%CO2. After maturation the cumulus cells were removed and the denuded oocytes were stained with 2% aceto-orcein to determine the nuclear oocyte status. The result showed that the rate of nuclear maturation in ITS-treated groups was significantly higher (P<0.05) than in the ITS-untreated group, were 38.14%a;  61,40%b and 71,55%b ,  respectively for medium without ITS,  5 ng/ml ITS and  10 ng/ml ITS.  This observation indicated that supplementation of ITS into culture medium was effectively enhance oocyte nuclear maturation in vitro.

Keywords:  oocyte, nuclear maturation, Insulin Transferin Selenium, in vitro maturation

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ISSN (Paper)2224-3208 ISSN (Online)2225-093X

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