Effects of Additional Crude Extract of Fig Fruit (Ficus carica L) into Tris Egg Yolk Based Extender on Quality of Buck Semen

Abstract

This research was to investigate the effect of crude extract of fig fruit (CEF2) added into tris egg yolk (TEY) based extender on sperm quality of Boer cross buck. The collected semen was divided into 8 treatments based on the level of CEF2 in TEY based extender (0% CEF2 to 7% CEF2, v/v, respectively). Progressively motile sperm, live sperm, plasma membrane integrity and normal sperm after re-concentration and preserved at 5ºC were assessed visually at 0 and every 24 hours for 7 consecutive days. The optimum concentration of CEF2 in TEY based extender to preserve progressively motile sperm was 6% when stored at 5ºC up to six days, and significantly higher (P<0.05) as compared to 0-3% and 7% CF3E. However, progressively motile sperm in 4%-6% CEF2 were non significant (P<0.05) each other. At day 6, additional 4-7% CEF2 into TEY based extender were not significantly different (P<0.05) from each other on the percentages of life sperms, but they were significantly higher compared to former extenders. There was a tendency that the highest percentages of plasma membranes integrity was in 6% of CEF2 and significantly higher (P<0.05) as compared to 0% and 1% of CEF2. Overall means of normal sperm in extender with 3-6% of CF3E were not significantly difference (P<0.05) each other except as compared to the former extenders. In contrast, the normal sperms indicated no significant difference (P<0.05) among treatments during this study. The concentration of MDA (ng/mL) in treatment extender was reduced when CEF2 was added to extender and the SOD concentration (u/ml) increased. It is concluded that the addition of CEF2 in TEY based extender provides a positive protection performance to the liquid buck semen quality.

Keywords: fig fruit extract, spermatozoa, quality, buck.