Comparative Assessment of Cytoprotective Effect of Aqueous Extract of Stem bark and Root of Azadirachta indica and Cassia italica on Oxidatively Stressed Human Erythrocytes

Abstract

The present investigation shows comparative assessment of aqueous stem bark extract and aqueous root extract of Azadirachta indica and Cassia italica, possesses an antioxidant activity that exhibits its protective action on lipid peroxidation and contributes to enhance its effect on cellular antioxidant defense. This activity thus proves to be helpful to offer adequate protection against oxidative damage in human erythrocytes. Exposure of erythrocytes to oxidative stress significantly increased lipid peroxidation and decreased the levels of glutathione and the antioxidant enzymes. When erythrocytes treated with aqueous stem bark extract (1.5mg/ml, 3mg/ml and 6mg/ml) Table 1: it could effectively and highly inhibit lipid peroxidation and enhance the activities of the antioxidant enzymes and glutathione content significantly. It could also be observed that when erythrocytes treated with aqueous root extract (1.5mg/ml, 3mg/ml and 6mg/ml) Table 2: Azadirachta indica and Cassia italica indicate less cytoprotective effect when compared with aqueous stem bark extract at the same concentration.

Keywords: Oxidative stress, Cytoprotection, Aqueous Stem Bark Extract, Aqueous Root Extract, Erythrocyte.