In Vitro Evaluation of Antibacterial Properties of Moringa oleifera, Dalbergia sissoo and Alstonia scholaris

Abstract

In this paper leaves of Moringa oleifera, Dalbergia sissoo and Alstonia scholaris in different solvent were subjected to antibacterial analysis against selected bacterial pathogens and phytochemical analysis was done. To investigate the  antibacterial activities of Drumstick tree (Moringa oleifera), Sheesham (Dalbergia  sissoo)  and  Dita  bark  (Alstonia   scholaris)  were  tested  against  bacterial  pathogens (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus). The dry crude sample extracts were tested for its antibacterial activities using ‘agar well diffusion technique’.  The solvents used were methanol, acetone, ethyl acetate and chloroform, compare to all, methanolic extracts showed best results with Staphylococcus aureus and Pseudomonas in case of Dalbergia sissoo; methanolic extracts also showed best results with Escherichia coli and Pseudomonas in case of Alstonia 1cholar while acetone and ethyl acetate showed best results with Staphylococcus aureus in case of Moringa oleifera. These samples were further taken to determine the MIC value.  The MIC value was determined using broth dilution method. Acetone and ethyl acetate extracts of Moringa oleifera were subjected to get MIC against Staphylococcus aureus and it was found to be 0.003mg/ml and 0.096mg/ml respectively. MIC values for methanolic extract of Dalbergia sissoo were 0.386 mg/ml for Staphylococcus aureus and 0.005 mg/ml for Pseudomonas aeruginosa. In case of methanolic extract of Alstonia scholaris MIC values were 41.67 mg/ml both for Pseudomonas aeruginosa and Escherichia coli. Important sources of phytochemicals of immense medicinal and pharmaceutical potential were present.

Keywords: Antibacterial activity, Methanol, acetone and ethyl acetate plant extract, MIC (Minimum Inhibitory Concentration) and phytochemical analysis.