Cytotoxic effect of the purified lectin from locally Isolate Acinetobacter baumannii on Hep-2 tumor cell line

Abstract

Lectins are glycoproteins of non-immune origin which have a wide range of application in medical field. In this study, a novel strain, Acinetobacter baumannii S₄ gave the highest production level of lectin by microscopic glass slide and microtiter plate methods and found that lectin agglutinated non-specifically red blood cells of human type A⁺, B⁺, AB⁺ and O⁺ also  the blood group O⁺ was the best among the other blood groups. Purification of the lectin was achieved by35% saturation ammonium sulfate followed by affinity ion-exchange chromatography on DEAE -cellulose column and  gel filtration chromatography Sephadex G-75 column with a yield of 36% and a purification fold of 4.66.This study showed that the purified lectin has a cytotoxic effects on cancer cell (Hep-2) as could be seen from their effects on inhibition percentage and the significant differences (p<0.05) which was observed by increasing the inhibition percentage as the concentration and time were increased. The higher level of inhibition(64%) was obtained at concentration56.25?g/ml after 72hour of exposure and when the concentration was increased more than56.25?g/ml, the inhibition rate was decreased.