Estimation of Interleukine -1 and Granulocyte Macrophage –Colony Stimulating Factors among Type a Hepatitis Patient and Autoimmune Hepatitis

Abstract

Hepatitis A Virus (HAV) is the most common cause of acute hepatitis worldwide. Although infection with Hepatitis A virus is often mild and asymptomatic in young children, the disease can be severe in adults .The aimed of this study was estimate serum cytokines levels of interleukin (IL-1) and Granulocyte macrophage\colony stimulating factors (GM\CSF) with Hepatitis A virus(HAV) , autoimmune hepatitis (AIH) and apparently healthy individuals as a control group. Forty  blood samples have been collected from HAV patients ,  31samples of  (AIH) patients ,   in addition to 24 apparently healthy individuals . The detection of (HAV),(IL-1) and  (GM\CSF)were estimated by Enzyme Linked  immuno sorbent assay (ELISA) method. The present study shows that the high frequency of disease in age 20-29 years most of them male more than female. The mean of the elevated IL-1 in serum of HAV, AIH and healthy control was 59.44 ± 27.23, 26.39 ± 13.35 and 4.97 ±2.78 respectively, while the mean of elevated GM-CSF in serum of the studied groups was 48.66±25.41, 12.4±3.74 and 1.71±0.88 respectively. IL-1 appears in 39 (97.5%) of HAV patients which was elevated in comparison with 27 (87.1%) AIH group. Level IL-1 among AIH was observed highly significantly elevated in comparison with healthy control (P<0.01). The elevated of GM-CSF in this study was showed that 40(100%)  patients with HAV were appeared of abnormal GM-CSF, while 31(100%)   AIH patients were observed for abnormal GM-CSF in AIH compared with healthy control.. The results indicating that highly significant relationship for the studied group (P<0.01). This study concluded that Sreum IL-1 and GM-CSF was elevated in both HAV and AIH as mediating acute inflammatory reactions and chemokine respectively compared with healthy controls.

Keywords: Interleukine -1, Granulocyte Macrophage –Colony Stimulating Factors ,type A Hepatitis autoimmune hepatitis