Anti- Lipase Activity of Some Plant Extracts on the Effectiveness of Lipase Enzyme Isolated and Purified from Pseudomonas Aeruginosa

Abstract

Bacterial isolates obtained from food.The isolates were grown on a selective medium agar that contained tween 80 or olive oil as the only source of carbon for detection of lipase activity. The organism grows optimally in the temperature of 40Cº and pH 7. The lipase present in the broth was purified with ammonium sulphate precipitation and filtration chromatography by Sephadex G-150 to obtain 0.108 folds pure enzymes.Anti lipase activity of each plant was determined by titrating the solution against 0.02M NaOH using phenolphthalein as an indicator.

Keywords: Purification, anti-lipase, plant extract, Ps. aeruginosa