Detection of Presence Fungi and Aflatoxines (AFB1 and AFG1 ) in Rice Samples Collected from Maysan Province

Abstract

This study aimed to examination the frequency and capacity of fungi and aflatoxins in rice samples stored collected from some houses in Maysan province, Iraq. The levels of aflatoxins produced by isolated Aspergillus flavus were also appraised. Fifty  rice samples  were investigated for fungal infection. The fungi were isolated and identified  were Cladosporium spp.,  Colletotrichum nymphaeae , Aspergillus flavus, A. niger, A. candidus,   Fusarium spp., F. oxysporum ,F. graminearum  ,  Penicillium spp.  , Mucor spp. , Trichoderma sp.,Rhizopus sp.,  Alternaria spp. , A. alternaria  and Chaetomium sp.. A.flavus recorded highest percentage of  frequency was 82%   flowed by A. niger and Penicillium spp., A. flavus recorded highest percentage of  presence was 90.3%   flowed by Cladosporium spp., 27  rice samples showed  ability to produce AFB1 ,while the AFG1 was produced from 22 rice samples . Results showed that it is wide difference of aflatoxins creation was evident amongst the experienced isolates of A. flavus expending, TLC technique, it was originate that 25 isolates, among 41 were able to produce AFB1  and 12 isolates were able to produce AFG1.

Keywords: aflatoxines ,AFB1,AFG1,A.flavus,rice, Maysan province,Iraq