Biocontrol of Wilt Disease on Pepper Using Endophytic Bacteria in Malang Indonesia

Abstract

Wilt disease is one important disease on pepper that cause by Ralstonia solanacearum, be potential limiting factor of product  and impact on economic country. Control of  it using some technique have not been success. Biocontrol of it using endophytic bacteria is one alternative control methods and support sustainable agriculture. The object of these experiment are to select of endophytic bacteria that was pepper bacterial wilt disease. There are 10 isolates successfully isolated and two isolates are Ps₁ and Ps₈ can inhibit of wilt disease based on antagonistic test in vitro using seed coat method and ananforming inhibition zone size 4-7 mm so ability suppress of wilt disease in vivo test in green house.  In vivo test in green house using pepper plant that 30 days age and aplicate of endophytic bacteria using root soak technique. The result show the two isolates are Ps₁ and Ps₈ have incubation period 15-16 days and significantly suppress disease incidence until 8.07-9.19%.

Keywords: wilt disease, endophytic bacteria, biocontrol