Experimental Study of the Role of Soft Laser in the Production of Probable Histological Alterations in the Architecture of Mouse Hepatocyte

Abstract

Our investigational present work was carried out using twenty four healthy male Swiss albino mice (age, 7-9 weeks; weight range, 24-28g). Mice were divided into 4 groups (n=6/group). The first group comprised control group (nonirradiated) of mice, and the other three groups served as irradiated groups. The upper right quadrant of the abdomen of the mice in the second, third, and fourth groups were irradiated by gallium arsenide laser (GaAs) for 15, 20, and 25 minutes once daily during the entire period of irradiation (7 days) respectively.

The laser caused various degrees of alterations in the architecture of the hepatocytes in both third and fourth irradiated groups of mice. In the third irradiated group of mice, the architecture of the hepatocyte revealed abundant alterations such as cleavage of the cytoplasm and multiplication of the nucleus, whereas in the fourth irradiated group of mice, the architecture of the hepatocyte revealed more abundant alterations such as cleavage of the cytoplasm and the multiplication of the nucleus is undistinguished because of the dark appearance of the cytoplasm as compared with the hepatocyte of the third irradiated group of mice. In the second irradiated group of mice, the architecture of the hepatocyte remained changeless as compared with the architecture of the hepatocyte in the first control group (nonirradiated) of mice.

Keywords: liver, laser, histological alterations, mice