Exploration of Natural Probiotics From Pineapple Peels (Ananas Comosus) as a Source of Feed Supplements for Ruminants

Abstract

The Aims of this research was conducted to evaluate, isolate and identificate of the pineapple peel as source of probiotics bacteria and identified of potentially to be used as supplement for feed of ruminant. The research were divided into three stages. The first stage were done to identification of nutrient and phytochemical contens of pineapple peel, the second stage were to isolation and identification of microbial bacteria from pineapple peel. The third stage were done carried out of the best carrier for microbial life (rice brand, crown, leaf and pineapple peel). Bacterial isolation was performed according to Hadioetomo method (1993). Identification of gene sequence were done PCR. The results ofthe research that in the first stage nutrient content of the pineapple peel there were 3.85% ash; 27.09% crude fiber; 1.15% crude fat; and 8.78% crude protein; 59.13% BETN. The Pineapples peel contained of phytochemicals there were 9 mg beta-carotene, alkoloid, flavonoids, triterpenoids, and phenolic. The results of the second stage of this research show that of the isolation of bacteria presented in the peel of pineapple had five bacterial isolates. The five bacterial isolates were than identified by DNA extracting with conducted molecular PCR. The results squencing showed that the bacterial of  Stenotrophomonas maltophilia strain W8-1 16S rRNA, Bacillus pumilus strain L 1 16S rRNA, Stenotrophomonas maltophilia strain Dh 16S rRNA, Stenotrophomonas maltophilia strain PBBC9 16S rRNA, Stenotrophomonas maltophilia strain PBBC9 16S rRNA. Fifth isolates selected one of them as a potential bacteria as probiotic bacteria is both the bacterial isolate Bacillus Pumilus strain L 1 16S rRNA. The Conclusion that the experiment carried out of Bacillus Pumilus bacteria strain L 1 16S rRNA were suitable to growing in the pH of 6 - 7.5, temperature of 37-50^oC and in the carrier of rice bran. Bacillus Pumilus bacteria strain L 1 16S rRNA gene to be used feed supplements for ruminant.

Keywords : Exploration, pineapple peel, probiotic, Supplement