Preparation Simplified Culture for Culturing Blastocystis Hominis Parasite

Abstract

Blastocystis hominis is an obligate and aerobic protozoa, a world waded distribution between human and animals. Anew simplified of culturing parasite is prepared. One hundred fresh positive stool samples were inoculated in tube containing a new media. This media contain simple compound like ringer solution, water rice cooked and don’t need serum add. Tubes were incubated at 37c°, culture's was examined after 24, 48 and 72hours, culture became positive after 24hr, and the number of parasite was (1830) while after 48hr was (1525) but after 72hr the numbers of parasite began decreasing and became (381.25). The multiform of parasite described but vacuolar and granular form more observed. This culture is a new, quick and easy method for culturing Blastocystis    homini also not require to add serum.

Keywords: Blastocystis hominis, vacuolar, granular, culture media.