Isolation and Identification of Lipase Producing Bacteria From Oil-contaminant Soil

Abstract

The novel lipase producing isolates from oil contaminated soil were selected. the isolates were collected from different sources . different solid media were used . the lipase activity of crude enzyme were determined. . eight isolates showed high lipase activity . the isolate pseudomonas aeruginosa strain showed maximum activity. the 16S rRNA gene sequence analysis were used to identify the eight isolates.

Keywords: Lipase producing bacteria, Isolation, Molecular identification