In Vitro Anthelmintic Activities of Crude Extraction of Parthenium Hysterophorus against Haemonchus Contortus

Abstract

This study was designed to evaluate anthelmintic activities of crude methanol extract of Parthenium hysterophorus against Haemonchus contortus. Parthenium hysterophorus leaves, stem, flower and roots crude extracts (1000mg/ml, 500mg/ml, and 250mg/ml) prepared in dimethyl sulfoxide then in vitro anthelmintic activity was evaluated against the Haemonchus contortus using Adult Motility Assay (AMA), Larvae Inhibition Test and Egg Hatch Test (EHT). Parthenium hysterophorus leaf crude extract show significant number (larvae L3) death in short time followed by flower when it is compared to other parts, positive and negative control groups (P <0.05). There is also significant number of adult death seen in groups treated with leaf and flower crude extracts with in short period of time when it is compared to other parts, even faster than the standard drug albendazole (P <0.05).  The crude extract exhibited dose and/or time dependent larvaecidal and adultcidal effects against H. contortus. The best larvaecidal and adultcidal was demonstrated by leaf, 100% mean mortality percentage seen within 2hours for larvae and 3hours adult. Methanolic extract of Parthenium hysterophorus possess anthelmintic activities especially the leave and flower work best against adult and larvae (L3). But further studies need on toxicity, and in vivo test

Keywords: Parthenium Hysterophorus, Haemonchus contortus, Anthelmintic Activities