Susceptibility of Escherichia Coli Isolated from Oligospermia Patient to Gongronema Latifolium Leaves Extracts

Abstract

Several studies has shown that the infection of the male genitourinary tract represent a significant healthcare problem and accounts for the major cases of oligospermia associated with microbial infections. The aim of this study was to evaluate the susceptibility of Escherichia coli isolated from oligospermia patient to Gongronema latifolium leaves extracts. A sample was collected from the hospital and subcultured on nutrient using streaking method, incubated at growth conditions. The phytochemical constituents of the leaves extracts of G. latifolium were determined quantitatively using spectrophotometric method. The antibacterial activity of the leaves extracts was carried out using agar well diffusion method. Tube dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using double fold serial dilutions at concentrations 400 mg/ml to 25 mg/ml.The bacterial isolate were characterized and identified using colony description and biochemical tests.The phytochemical analysis of G. latifolium leaves extracts revealed the presence of alkaloids, saponins, flavonoids, steroids, cardiac glycosides, phenolics and tannins. The ethanolic extracts (17.00 mm) of G. latifoliumshowed more activity than aqueous extracts (6.00 mm) and their activities differed significantly (P<0.05) from Ciprofloxacin (20.33 mm). The MIC (200 mg/ml, 400 mg/ml and MBC (400 mg/ml, Nil) values revealed that the inhibitory and cidal activities of the ethanolic and aqueous extracts. This study has shown that G. latifolium leaves extracts showed pronounced activity against E.coli isolated from oligospermic patient and otherwise extract proved to be more effective.