Molecular Detection and Identification of Aspergillus fumigates from Goats in Waist Governorate of Iraq

Abstract

A total of 100 samples of goats include 50 samples were collected from each tracheal swabs, and vaginal swabs from slaughter house and herds of goats in Waist province. During these studied, molds were isolated and identification depending on the cultural characteristic, microscopic feature and conventional Polymerase chain reaction (PCR). This study showed increase in the isolation of oppurtusinstic molds with increase age of animals, which observation from this study on goats found highest percentage of molds in those with  ages between 3-5 years, while decrease percentage in animals that ages less than six months. The results showed significant differences (P<0.01) in molds isolation with increases animals ages in goats. Through study found Aspergillus spp. were predominant species. The most isolate of opportunistic molds were from tracheal swabs at percentage 63.6%, were significantly different (P<0.01) than vaginal swabs. The results of molds isolation showed the A.fumigatus and A.niger, were more isolated at percentage 40.9% and 22.8% respectively, followed by A.terrus (18.2%), A.flavus (9.1%), Aspergillus spp (4.5%) respectively, also isolated Penicillium spp. at percentage (4.5%).   Showed result Significant differences (P<0.01) were found in isolation of molds between tracheal swabs, and vaginal swabs. The results of the PCR amplification of the rRNA gene showed that, this gene was present in 9 samples out 22 positive samples which isolation from the goats with a PCR product size of approximated  380 bp, while 13 samples out 22 positive samples showed negative results for the presence of this gene as indicated by the absence of the PCR products. The conclusion of present investigation indicated Aspergillus fumigates the most dominant molds isolated from tracheal swabs in goats, and this may be indicating a high contamination and exposure to Asperigllus spores. Molecular methods have revealed the important diversity of Aspergillus species within these action Fumigati.

Keywords: A.fumigatus, Goats in Waist Governorate, Polymerase chain reaction (PCR), rRNA gene