In Vitro Regeneration of Plectranthus Edulis (Vatke) from Leaf Derived Callus

Abstract

Plectranthus edulis (Vatke) Agnew is a tuber-bearing food in Ethiopia and used as a traditional medicine to treat different diseases. Vegetative propagated plants are mostly affected by different diseases. The establishment of in vitro plant regeneration system in P. eduils is of potential importance to genetically improve this plant using different biotechnological approaches including genome editing and genetic transformation. Best calli (100 %) were obtained on MS medium supplemented with 1.5 mg/l NAA+1.0 mg/l BAP and 2.0 mg/l NAA+0.5 mg/l BAP. For multiplication, maximum (58.68±6.57) mean numbers of shoots were obtained on 0.5 mg/l BAP in combination with 0.4 mg/l GA₃. Best rooting (10.15±0.95) was obtained on MS medium supplemented with 2.0 mg/l IBA. After a month, all in vitro rooted and 78 % of microshoots were survived in the glasshouse. This protocol can be used to improve this plant through in vitro screening, genome editing and genetic transformation.

Keywords: callus induction, growth regulator, organogenesis, regeneration.

DOI: 10.7176/JBAH/9-5-03

Publication date:March 31^st 2019