Frequency and Antimicrobial Susceptibility of the Bacteria Isolated from Blood Cultures of Neonatal

Abstract

The present study was carried out to determine the prevalence of bacterial isolates responsible for blood   stream infection (BSI) among neonatal inpatients at Baghdad Teaching Hospital / Baghdad and to also get an updated knowledge about their antibiotic resistance pattern during the period extended from 1 October till 31 December, 2018. All blood cultures were done by BacT/ALERT 3D device (Bio-Merieux, France). Identification of pathogenic bacteria at species level was done by using API system (Bio-Merieux, France) and the antimicrobial susceptibility testing was done by Kirby-Bauer method. A total of 90 blood cultures were received from neonatal suspected with blood stream infections. Out of 36 positive blood cultures, the incidence of positive blood cultures was higher among males 22(61.1%) than of females 14(38.9%). Out of 36 positive blood cultures, the isolation rate of Gram positive and Gram negative isolates was 25 (69.4%) and 11(30.6%), respectively. Coagulase negative Staphylococci (CoNS) was the most frequently isolated bacteria in blood cultures, 17 (47.22%) isolates. Our study also showed that Klebsiella pneumoniae was the most frequently isolated Gram negative bacteria in blood specimens from neonatal inpatients 6(16.67%). The most effective antibiotic on Gram-positive isolates was Vancomycin, while for Gram negative bacteria the most effective antibiotic was Imipenem.

Keywords: Blood stream infection, Antimicrobial susceptibility, Neonatal blood cultures and Bacterial isolates

DOI: 10.7176/JBAH/9-14-05

Publication date:July 31^st 2019