Prevalence of Methicillin Resistant Staphylococcus aureus in Dairy Farms in Meki Town

Abstract

The current study was conducted with the objective of identifying Methicillin resistant Staphylococcus aureus from mastitis positive quarters of dairy cows based on phenotypic screening method. Staphylococcus aureus was first identified by bacteriological culture and biochemical tests. Then after, identification of the isolates for Methicillin resistance was performed using growth inhibition by Cefoxitin Disc by disc diffusion technique on Mueller Hinton Agar. In the study area, an overall prevalence of Methicillin resistant S. aureus (MRSA) was revealed to be 32%. Amongst the isolates of Staphylococcus aureus, statistically invariable (p>0.05) methicillin resistant Staphylococcus aureus was found in 38.9% of clinical and 28.1% of subclinical mastitic samples. It was found evident that Methicillin resistant S. aureus was prevalent in the study area regardless of sensitivity of identification used for the study. In connection with this, consideration for antibiotics of treatment and mastitis health care management is valuable option to control emergence and spread of the bacteria in dairy herds.

Keywords: Prevalence, S. aureus, Meki, dairy farm, MRSA

DOI: 10.7176/JBAH/9-23-03

Publication date: December 31^st 2019