Hydrolysis of Tuber Peels and Sorghum Chaff by Cellulolytic Culture Filtrates of Aspergillus Niger AC4 Isolated from Agricultural Waste Dumpsites
Abstract
Six of the twenty strains of Aspergillus niger isolated from tuber peels and sorghum chaff collected from twenty two agricultural waste dumpsites show strong capacity for producing cellulase enzyme using PDA plate supplemented with 2% (w/v) carboxylmethylcellulase incubated at room temperature. Cellulase production was detected by staining the plates with 2% congo red solution and then measuring the zone of clearance around the fungal colonies on the plates. Aspergillus niger AC4 produced the largest zone of clearance (3.63cm) on the PDA congo red stained plate and also gave the highest cellulase activity (240U/ml) on hydrolysis hence it was selected for further studies. Culture filtrates of the Aspergillus nigerAC4 had optima temperature, concentration and pH at 35oC, 4% and 5.0 respectively. Culture filtrates of Aspergillus nigerAC4 was also used to hydrolyze yam peel, cocoyam peel, sweet potato peel, sorghum chaff and carboxylmethylcellulase (CMC) in comparison. Hydrolysis was significantly dependent on cellulose source and length of incubation (P<0.05). cellulose activities differ significantly in the cellulose sources ranging from 240U/ml in cassava peel to 54U/ml in sorghum chaff. It is concluded that cassava peel might be a better substrate for the production of cellulase by Aspergillus niger than commercial carboxymethlcellulase (CMC).
Key words: Tuber Peels, Sorghum Chaff, cellulolytic filtrate and hydrolysis.
To list your conference here. Please contact the administrator of this platform.
Paper submission email: JBAH@iiste.org
ISSN (Paper)2224-3208 ISSN (Online)2225-093X
Please add our address "contact@iiste.org" into your email contact list.
This journal follows ISO 9001 management standard and licensed under a Creative Commons Attribution 3.0 License.
Copyright © www.iiste.org