An Alternative to Increase Accumulation of Phenolic Compounds in Grapevine Callus Cultures: Chemical Mutagen Applications

Abstract

This research was carried out to investigate the effects of chemical mutagens on producing phenolic compounds in calli obtained from Royal grape variety. Petioles were cultured in B5 medium (0.5 mg/L benzyl amino purine and 0.5 mg/L indole acetic acid) to obtain callus. Calli were subcultured in the same nutrient medium three times at one month intervals. In the fourth subculture, the calli were transferred to media with the same composition containing ethyl methanesulfonate, sodium azide, azacitidine and acridine orange in three different doses (0.5 mM, 1.0 mM, and 2.0 mM) and cultured in these mediums for 60, 120 and 180 minutes and then they were cultured for four weeks in mutagen-free nutrient mediums with the same content as their previous mediums. After that total phenolic compound, total flavanol, total flavonol and anthocyanin analyzes were performed. As a result, all mutagens applied are effective in increasing the production of phenolic compounds depending on the dose and time.

Keywords: Grapevine, Chemical mutagen, Phenolic compounds, Anthocyanin.

DOI: 10.7176/JBAH/11-22-05

Publication date: November 30^th 2021