Effect of Mineral Acids on Rooting Response of Aging Mung Bean (Phaseolus aureus Roxb. ) Cuttings via Indole Acetic Acid Level

Abstract

The influence of sulfuric acid (H₂SO₄) as a strong mineral acid on rooting response of fresh and aging mung bean cuttings has been studied on the level of (IAA) . The data revealed significant increase in rooting response of aged cuttings      ( for 3 days in d/H₂O ) with (0.001 and 0.01%) concentrations of H₂SO₄ solution and highly significant increase in rooting response of aged cuttings (for 3 days in H₂SO₄ solution) with (0.0001 , 0.001 and 0.01%) concentration , While highly concentration (0.1 and 0.5 %) revealed highly significant decrease in rooting response compared to control (d/H₂O). Quantitative estimation of IAA by spectrophotometric method as indicators for oxidative processes that occur during aging phenomenon verified a highly significant increase of IAA content in hypocotyles of aged cuttings in optimal concentration of H₂SO₄ .

Key words: Aging, IAA biosynthesis, Rooting response, Mineral acids, Macronutrients, Stem cuttings.