Low Cost Alternative Method to Monitor the Electrophoretic Bands Substitution of the Positively Charged Rabbits Seminal Fluids

Abstract

This paper aimed to evaluate the significance of a non-cost effective double dye technique in visualizing any DNA-seminal proteins interactions. After collecting rabbit’s ejaculate and removing sperm cells, ion exchange chromatography was performed to separate seminal proteins on the basis of their charge. Positively charged seminal proteins were eluted, lyophilized, and involved in this study. After incubating this eluted group with a standard reporter DNA, band shift assay was applied on this group. The results were compared. According to this study, we demonstrated the necessity of utilizing a double staining technique for the same band shift gel in order to ensure weather real or false band substitutions were obtained.

Keywords : Rabbits seminal fluids ,DNA , Electrophoresis