Identification and purification and antimicrobial activity of alkaloid from Peganum harmale (L), a medicinal Plant.

Abstract

The sample from seed of Peganum harmala plant tree was extracted in hexane, chloroform, acetone and methanol and separated by thin layer chromatography on silica gel plate by using chloroform : Methanol (85:15) solvent mixture and detected under the ultraviolet light. Maximum bands were detected on TLC of methanol extract. Those bands were scraped and the purification of the single band of interest molecule was done by preparative silica gel column chromatography. Antimicrobial activity of single band isolated was tested against standard ATCC (American Type Culture Collection) strain of E.coli (25922), Klebseilla, Staphylococcus aureus (25923), Pseudomonas aeruginosa (27853), S. pneumonia (6305) using Disc diffusion method and Punch Plate technique. The Purified fraction of alkaloid show zone of growth inhibition with 10mm on ATCC Staphylococcus aureus. The sensitivity test of water extract was showing effect mostly against Staphylococcus aureus and E.coli.