In Vitro Multiple Shoot, Root and Callus Induction from Different Explants in Tomato (Lycopersicon Esculentum Mill)

Abstract

Tomato (Lycopersicon esculentum Mill) is an important crop that plays an important role in Pakistan economy. There are three commercial varieties of tomato i.e. Roma, Moneymaker and Nagina grown all over the country. In vitro culture response was assessed in these three varieties for optimum callus induction and plantlet regeneration. Results showed that under in vitro condition seed germination increased with the passage of time. Statistical analysis of data regarding callus induction from cotyledon leaf and hypocotyls explants showed highly significant results for three varieties. When cotyledon was used as explants, highest callus induction percentage was observed in cultivar Moneymaker (91.4%) containing MS medium supplemented with NAA+BAP 1+ 2 mg/L. Variety Moneymaker was found better for callus induction when cotyledon was used as explant.