Evaluation of Some Biomarker and Genetic Marker in Myocardial Infraction Patients

Abstract

Acute coronary syndrome (ACS) consists of unstable angina pectoris, non-ST segment elevation myocardial infarct ion and ST segment elevate ion myocardial infarction.  The aim of the study was to compare the vibration  some hormones levels in the Acute coronary syndrome patients and control groups and This study aimed to investigate the association between two single nucleotide polymorphisms (?1562C>T, of the MMP-9 gene in patients with ACS in the Iraq population.

Subjects and methods: This study was conducted between / mrjan teaching hospital in Babylon Province between November 2012- Aprial 2013and it was carried out at the coronary care unit  / in Babylon province/Iraq .  This patient-control study was composed of 60 ACS patients with age 53.38 ± 9.51 and 30control withe age  51.43 ± 7.81subjects, The present study is divided into two main parts: physio-biochemical and molecular parts. The physiological part involved hormonal assay( troponin and endothelin-1) while molecular part included The genotypes of the  selected SNPs were determined by the method of polymerase chain reaction and restriction fragment length polymorphism (RFLP-PCR). The relationship between the polymorphism of the MMP-9 gene and the severity of Acute coronary syndrome with negative medical history.

The result of this study showed that patients with Acute coronary syndrome both males and females had significantly troponin and elevated Endothelin -1 in patient than control<0.001**(**p value ? 0.01 was significant).Analysis of the SNPs showed that the frequency of CT and TT genotypes in patients with ACS was significantly higher than that in the control group (ACS vs. controls; CT+TT: (85%Vs 36%).

Keywords:single nucleotide polymorphisms, troponin, endotheline, MMP-9