Isolation of Cellulose Degrading Bacteria from Indoor, Outdoor

Abstract

Cellulose is the most abundant organic polymer on Earth and is an important structural component of the primary cell wall of green plants. Cellulose should be degraded and converted to into soil and recycled through the activity of microorganisms. Bacteria play important role to degrade cellulose for their energy requirements to sustain their life. The current study was initiated to isolate and identify Cellulose Degrading Bacteria from indoor, outdoor and Cellulose rich soil by using Methyl Cellulose Agar media. Three Petri dishes with three replication both heat boiled and autoclaved containing culture media, from indoor air, outdoor air and soil has been tested for their effective Cellulose degrading bacterial growth ability. Cellulose degrading bacteria has been isolated with providing Methyl Cellulose only as carbon source. The Autoclaved outdoor air shown best result with fast growth and many bacterial colony following indoor and soil samples. These cellulose degrading bacteria has been tested for their Gram test, Catalase test, motility test and carbohydrate consumption tests and shown Gram positive as well as the rest tests were also positive results. Therefore, isolation and application of cellulose degrading bacteria will have great benefits in terms of degrading pollutants from the environment.

Keywords:Cellulose, Cellulose degrading bacteria, Methyl Cellulose Agar

DOI: 10.7176/JEES/11-11-01

Publication date: November 30^th 2021